Figure 8
From: Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells
Profiling of patient derived ovarian cancer cells. Ovarian cancer cells were isolated from either tumor specimens or ascites samples of naïve patients diagnosed with ovarian cancer as described in the Materials and Methods. An equal number of cells were plated in multi-well Seahorse Biosciences XF96 plate (Boston, MA) and (A) oxygen consumption rate (OCR) and ECAR was measured under challenge by various metabolic regulators as described earlier. The ECAR profile injections were (1) glucose (fuel for glycolysis), (2) oligomycin (an ATP synthase blocker) and (3) 2DG (an inhibitor of glycolysis). OCR profile injections were (1) oligomycin (an ATP synthase inhibitor), (2) FCCP (an electron transport chain uncoupler) and (3) rotenone (an inhibitor of electron transport chain) injections. (B) Range of basal OCR and maximum capacity in the patient derived ovarian cancer cells. (C) Range of basal ECAR and glycolytic capacity in the patient derived ovarian cancer cells. (D) Ratio of basal ECAR and OCR represented as percent to represent the bioenergetic preference of a cell. (E) Positive correlation between ATP-linked respiration and spare glycolytic capacity. Seahorse experiments were replicated twice in triplicates. Abbreviations: ATP: adenosine triphosphatase; 2DG: 2-deoxyglucose; FCCP: carbonylcyanide-p-trifluoromethoxyphenyl hydrazine; OCR: Oxygen Consumption Rate; ECAR: Extracellular Acidification Rate.
