Figure 2
From: Ccdc3: A New P63 Target Involved in Regulation Of Liver Lipid Metabolism
CCDC3 protein level is induced by overexpression of TAp63 and decreased by knockdown or knockout of TAp63. (a,b) WB analysis showing that overexpression of TAp63 in H1299 cells (a) and HACAT cells (b) can induce CCDC3 protein level, while p40 inhibits CCDC3 expression. (c) p63 knockdown decrease CCDC3 protein level in HaCat cells. (d) WB analysis showing CCDC3 protein level is lower in TAp63−/− MEF cells than that in WT MEF cells. (e) TAp63−/− MEF cells infected with GFP (control), TAp63, or p40 adenovirus as indicated followed by WB analysis with indicated antibodies. (f) IF staining for CCDC3 (red) and DAPI (blue) using the brown adipose tissue from the indicated WT and TAp63−/− mice. (g) Quantification of the intensity of CCDC3 staining from Fig. 2f. Densitometry analysis is labeled on top of each band.
