Figure 5
From: Ccdc3: A New P63 Target Involved in Regulation Of Liver Lipid Metabolism
CCDC3 is ubiquitously overexpressed in transgenic mice after CMV-Cre mediated DNA recombination. (a) Schematic showing the construct of CCDC3 transgenic mice. Flag-mouse CCDC3 was placed downstream of a loxP-flanked Stop cassette and linked to a GFP which transcribed from IRES promoter. Following Cre-mediated excision of Stop cassette, Flag-ccdc3 is expressed. (b) Representative images of GFP expression in a whole E11.5 embryo and an isolated heart from a two days old mouse, taken under an anatomic microscope, confirming the global expression of CCDC3 in TG mice. (c) WB analysis confirms the CCDC3 protein express in E11.5 embryos and heart tissues of TG mice. (d) qRT-PCR verifies the expression of CCDC3 in different tissues of adult TG mice. (e) ELISA confirms the high level of CCDC3 in the blood serum of adult TG mice. Data represent the mean ± SD. *P < 0.05.
