Figure 6

Overexpression of CCDC3 alleviates glucose intolerant, insulin resistant and fatty liver formation. (a,b) Glucose tolerance test (a) and Insulin intolerance test (b) were performed using 5-month-old WT and CCDC3 TG mice on high-fat Diet (HFD) for 4 months, each point on the graph indicates the level of glucose in the blood at the indicated time point on the x-axis. (c) Representative photographs of fatty liver from mice in groups on HFD (upper images), and CCDC3 immuoflurorescence staining images from the same liver sections (lower images, polyclonal antibodies again CCDC3(Red) merged with DAPI(blue). (d) Representative Images of mouse liver sections after oil red O staining, × 20 magnification. Red staining indicates lipid deposits. (e) Hepatic triglyceride levels. *P < 0.05. (f) Representative images of F4/80 immunofluorescence staining of mouse liver cryosections. Polyclonal antibodies against F4/80 (Green) merged with DAPI (blue). (g) mRNA expression of different genes in mouse livers as indicated (n = 3 for each group). (h) Q-RT-PCR analysis of mRNA expression of genes involved in lipogenic and fatty acid oxidation in mouse livers (n = 8). *P < 0.05, **P < 0.01.