Figure 5
From: Periostin-binding DNA aptamer treatment attenuates renal fibrosis under diabetic conditions
Renal periostin, fibronectin and type I collagen expression in diabetes mellitus (DM) and non-DM mice treated with or without a periostin-binding DNA aptamer (PA). (A) Periostin, fibronectin and type I collagen mRNA levels in the kidney (n = 8). Periostin, fibronectin and type I collagen mRNA expressions were significantly increased in uninephrectomized STZ-induced DM (UNXSTZ) and db/db mice compared with the control and db/m mice, respectively. These increases were significantly abrogated by PA administration (500 µg/kg/d). (B) Representative western blotting analysis of periostin, fibronectin, and type I collagen protein levels (a representative of four blots). The protein levels of periostin, fibronectin, and type I collagen were significantly increased in UNXSTZ and db/db mice compared with the control and db/m mice, respectively. The PA treatment significantly ameliorated these increases in the DM mice. Uncropped scans are presented in Supplementary Figure 5. C, control; PA, periostin-binding DNA aptamer; UNXSTX, uninephrectomized STZ-induced DM mice. *P < 0.05 vs. C; **P < 0.05 vs. UNXSTX; #P < 0.05 vs. db/m; †P < 0.05 vs. db/db.
