Figure 6

The tumorigenicity and EMT phenotype were decreased by interfering HIF1α expression in hypoxia condition. (A) Tumorigenicity of SKOV3 cells was detected after 24 h HIF-1α siRNA treatment in hypoxia condition. Pictures were taken 4 weeks after subcutaneous injection. (B) Quantitative analysis of tumor volume in each group is listed. Data of three replicates are shown as means (±SD). ***P < 0.001. (C)The expression of HIF-1α was detected by RT-PCR in tumor. Data of three replicates are shown as means (±SD). ***P < 0.001. (D and E) RT-PCR was performed to detect the expression of Vimentin and E-cadherin in ovarian cancer cells SKOV3 and HO8910 after 24 h of HIF-1α siRNA treatment in hypoxia condition. GAPDH was used as an internal reference. Control siRNA was used as control. *p < 0.05, **p < 0.01, ***p < 0.001.