Figure 7

HIF-1α unpreguated SIRT1 expression in ovarian cancer cells. (A and B) RT-PCR was performed to detect the expression of SIRT1 in ovarian cancer cells SKOV3 after 24 h of hypoxia treatment or HIF-1α siRNA treatment in hypoxia condition. GAPDH was used as an internal reference. Control siRNA was used as control. **p < 0.01, ***p < 0.001. (C and D) Western blot was used to detect the expression of SIRT1 in ovarian cancer cells SKOV3 after 24 h of hypoxia treatment or HIF-1α siRNA treatment in hypoxia condition. GAPDH was used as an internal reference. Control siRNA was used as control.