Figure 2 | Scientific Reports

Figure 2

From: Expansion of cytotoxic natural killer cells using irradiated autologous peripheral blood mononuclear cells and anti-CD16 antibody

Figure 2

A combination of the anti-CD16 (αCD16) mAb with irradiated autologous PBMCs (IrAPs) strongly induces NK cell expansion. NK cells isolated from PBMCs were expanded under various culture conditions described in the Methods. (A) Schematic representation for expansion procedure of highly cytotoxic NK cell by the combination of the αCD16 mAb and IrAPs. (B) NK cell proliferation was analyzed using the EZ-Cytox Cell viability assay kit on day 5. (C) The fold expansion of NK cells was determined before (day 0) and after (days 14 and 21) NK cell expansion. The graphs show mean ± SD of five donors. The assay was conducted in triplicate for each donor. The statistical significance was determined by one-way ANOVA. # P < 0.05, ## P < 0.005, ### P < 0.0005 (#NK alone versus other groups). *P < 0.05, ***P < 0.0005 (*NK + αCD16 versus other groups). @@ P < 0.005 (@NK + IrAPs versus NK + αCD16 + IrAPs). ※ P < 0.05, ※※※ P < 0.0005 (※NK + αCD16 + IrAPs versus other groups).

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