Figure 6

Effect of EP3^−/− on responses evoked by PGI₂ in NOS-inhibited mouse abdominal aortas. (A) and (B) Responses evoked by the TP agonist U46619 (A) and PGI₂ (B) in WT and EP3^−/− abdominal aortas. (A) representative traces with summarized values showing the response evoked by PGI₂ in WT (top) or EP3^−/− abdominal aortas (bottom) with the presence of TP antagonist SQ29548 (10 μM; SQ). In A–C, n = 5 for each; ^** P < 0.01 vs. WT. (D) Genotyping showing the difference in the PCR product of EP3 gene between WT and EP3^−/− mice. M: size marker.