Figure 2
From: A novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS437
(a) The TEM image and approximate induced prophage measurement. Crude induced lysate was filtered with 0.22-µm-pore-size filter and centrifuged to pellet the cell derbies, then store in −20 °C for one night prior imaging. Image shows a typical Myoviridae family, the image also shows induced ɸ437 (red arrow) and ɸ437 that has lost its capsid structure (black arrow). The bar represents 100 nm. (b) genome sequence of ɸ437. Red arrows indicate phage lysis and lysogenic genes; blue arrows indicate phage structural genes (tail, capsid and fiber); green arrows indicate replication, recombination, repressor and phage related genes; gray arrows indicate hypothetical proteins. The black knobs indicate ρ-independent terminator and the bent arrows indicate putative promoters. The star indicates phage tRNA. (c) the attachment sites attP and attB of ɸ437. The att sites were analyzed using motif-finding tools MEME49. The attachment sites on the tRNA P. terrae BS437 (attB) and ɸ437 (attP) are shown.
