Figure 4 | Scientific Reports

Figure 4

From: Improved efficacy and in vivo cellular properties of human embryonic stem cell derivative in a preclinical model of bladder pain syndrome

Figure 4

The effect of M-MSC injection on visceral hypersensitivty in HCl-IC model rats. (a) Hematoxylin and eosin (H & E) staining in the indicated bladder tissues (magnification ×200, scale bar = 100 μm). Nuclei were stained with Mayer’s hematoxylin. Arrows indicate the infiltrated mast cells. N (black arrowhead) and m (white arrowhead) indicate the nerve and muscle fibers, respectively. (b) Quantification of the mast cells infiltrated around the nerve fibers (n = 15). (c) RQ-PCR analyses of expression levels of Ngf and the genes associated with visceral hypersensitiviy in the indicated bladder tissues 1 week after the injection of 1 × 106 M-MSCs or PBS vehicle into HCl-IC animals. Expression is presented as % Gapdh (n = 10). All data are presented as the mean ± SEM, *p < 0.05, **p < 0.01, Bonferroni post-test.

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