Figure 10

Assessing the number of full-length protein-coding transcripts within transcriptome assemblies, with UniProtKB/SwissProt BLAST hits (e-value ≤ 1 × 10⁻²⁰) categorised by length of alignment (nucleotides) with the UniProtKB/SwissProt reference transcript. Transcripts are considered ‘full-length’ if they are >90% of the reference transcript length. SOAPdenovo-Trans (3): moderate filtering of poor quality read pairs; Trinity (1): light filtering of poor quality read pairs, combined with in silico normalization; (2): moderate filtering of poor quality read pairs; (3): light filtering of poor quality read pairs, combined with SEECER error correction; (4) Trinity v2.3.2 after moderate filtering of poor quality read pairs; tr2aacds merged assemblies (v1): pre-v2.3.2 Trinity; (v2) including v2.3.2 Trinity output.