Figure 6
From: Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin
NCL expression affects SREBP1 accumulation and localization. (A) The level of SREBP1 protein was determined by western blot in cells transfected with control siRNA or with siRNA for NCL. Total extract were used and loaded onto a 10% SDS-PAGE. (B) Quantification of the expression of NCL and SREBP1 in untransfected cells (UT), and in cells transfected with control or NCL siRNA. Quantifications represent an average of 3 independent experiments. (C) Immuno-localization of SREBP1 in cells transfected with control or NCL siRNA. 96 h after transfection, cells were process for immunofluorescence with anti-SREBP1 antibody and with DAPI. Cells were then observed with confocal microscope (Zeiss LSM510).
