Figure 4
From: Excess α-synuclein compromises phagocytosis in iPSC-derived macrophages
pMac take up fibrillar and monomeric αS. (A) 10 µg/ml Oregon Green 488-labelled fibrillar αS was added to pMac (imaged every 5 mins, 18 hrs). Frames capture one selected phagocytosis event (scale bar = 50 µm). (B) Intracellular αS levels following incubation with monomeric αS (FACs), normalized to mean endogenous αS levels in untreated pMac. (C) pMac incubated with monomeric αS, with or without Cytocholasin D (FACs as in (B)). (D) pMac incubated with fluorescent αS fibrils, with and without Cytocholasin D (FACs), MFI fluorescent αS divided by MFI medium only. (E) as per (D) but lower dose and shorter time. (F) TNFα levels in pMac supernatants measured 24 hrs after monomeric or fibrillar αS or LPS. Statistical analyses: (C,D,E) two-tailed t-test; (B,F) one way ANOVA with Dunnett’s multiple comparisons test. Also see Figure S4D–F.
