Figure 6
Res inhibited activation of NF-κB by PRV through inhibiting IκBα degradation and RelA nuclear translocation. PK-15 cells were infected with or without PRV at an MOI = 1 and with or without Res (65.72 μM). Expression of NF-κB signalling pathway proteins, IκBα, RelA and β-actin in cytoplasmic extracts and RelA and Lamin B1 in nuclear extracts were determined by Western blot at 0, 1, 2, 4, 6, 8, 10 and 12 hpi (there was no significant difference from 0 to 4 hpi in all groups, data not shown). Values are means ± SD (n = 3). Correlation analyses were evaluated by Pearson r2, ns: p > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. normal cells.
