Figure 3
From: MicroRNA-449c-5p inhibits osteogenic differentiation of human VICs through Smad4-mediated pathway
Overexpression of miR-449c-5p inhibits VICs osteogenic differentiation while downexpression of miR-449c-5p promotes the process. (a) qRT-PCR analysis of miR-449c-5p expression in VICs transfected with miR-449c-5p mimic or inhibitor at day 2. (b) qRT-PCR analysis of Runx2 expression at day 7 after osteogenic differentiation. (c) ALP activity at day 14 after osteogenic differentiation. (d) Alizarin red staining at day 14 after osteogenic differentiation. (e) Quantitative analysis of alizarin red staining. n = 3 in each group. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01.
