Figure 4
From: MicroRNA-449c-5p inhibits osteogenic differentiation of human VICs through Smad4-mediated pathway
MiR-449c-5p directly targets Smad4. (a) Schematic of the putative miR-449c-5p target site in human Smad4 3′-UTR and the eleven mutated nucleotides are colored red. (b) Dual-luciferase assay after transfection of M-miR-449c-5p, NC-miR-449c-5p with wild-type Smad4 3′-UTR or mutant Smad4 3′UTR. n = 3 in each group. Data are presented as the mean ± SD. *P < 0.05.
