Figure 5
From: MicroRNA-449c-5p inhibits osteogenic differentiation of human VICs through Smad4-mediated pathway
Regulation of Smad4 by miR-449c-5p during VICs osteogenic differentiation. (a) qRT-PCR analysis of Smad4 relative mRNA expression at day 7 after osteogenic differentiation. (b,c) Western blot analysis of Smad4 at day 14 after osteogenic differentiation. (d,e) Western blot further confirmed Smad4 knockdown could block the effect of miR-449c-5p inhibitor during VICs osteogenic differentiation. n = 3 in each group. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01.
