Table 2 Primers used in the research.
From: MicroRNA-449c-5p inhibits osteogenic differentiation of human VICs through Smad4-mediated pathway
Gene name | Primer sequences |
|---|---|
Smad4 | Forward: GATACGTGGACCCTTCTGGA |
Reverse: CCTTTGCCTATGTGCAACCT | |
Runx2 | Forward: GGACGAGGCAAGAGTTTCAC |
Reverse: TTCCCGAGGTCCATCTACTG | |
GAPDH | Forward: AGCCACATCGCTCAGACAC |
Reverse: TGGACTCCACGACGTACTC | |
U6 | Forward: CTCGCTCGGCAGAACA |
Reverse: AACGCTTCACGAATTTGCGT | |
WT Smad4 | Forward:TAGAGCTCTCCTGAGAGCTTGGTTGTTAATC |
Reverse: ACGTCTAGACAAGTATGGCTCTCCTTAAGC | |
MutantSmad4 | Forward: TTGTTTAAACGAGCTCTCCTGAGAG |
Reverse: GTTAGTCTATTGGCTGTCATTATCTTTG | |
Forward: GCCAATAGACTAACTATAACCTTGC | |
Reverse: GACTCTAGACAAGTATGGCTCTCCTT |
