Table 2 Cultivation media.
From: Eukaryotic transporters for hydroxyderivatives of benzoic acid
Medium | Composition |
|---|---|
DMEM + FSB | Dulbecco’s Modified Eagle’s Medium (Lonza), 10% (w/v) heat-inactivated fetal bovine serum (EuroClone) |
SD | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 2% (w/v) glucose |
SD + MPA | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 2% (w/v) glucose, 200 μg/ml mycophenolic acid (Sigma-Aldrich) |
SD1% | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 1% (w/v) glucose |
SD1% + FBS | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 1% (w/v) glucose, 10% (v/v) heat-inactivated fetal bovine serum (EuroClone) |
S3OH | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 10 mM 3-hydroxybenzoate |
S4OH | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 10 mM 4-hydroxybenzoate |
S2,4diOH | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 10 mM 2,4-dihydroxybenzoate (β-resorcylate) |
S2,5diOH | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 10 mM 2,5-dihydroxybenzoate (gentisate) |
S3,4diOH | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 10 mM 3,4-dihydroxybenzoate (protocatechuate) |
SHyd | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 10 mM hydroquinone |
SRes | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 10 mM resorcinol |
SEtOH | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 2% (v/v) ethanol |
SGal + MPA | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 2% (w/v) galactose, 200 μg/ml mycophenolic acid |
SGly | 0.67% (w/v) yeast nitrogen base w/o amino acids (Difco), 3% (w/v) glycerol |
YCB + BSA | 1.17% (w/v) yeast carbon base (Difco), 2% (w/v) bovine serum albumin |
YPD | 1% (w/v) yeast extract, 1% (w/v) peptone, 0.5% (w/v) glucose |
