Figure 2
hASC and hASC-CM treatments reduce allodynia in STZ mice. (a–f) Effects of i.v. hASC (1 × 106) or hASC-CM (obtained from 2 × 106 cells) treatments on mechanical allodynia in STZ mice. (a,b) Mice received a single hASC or hASC-CM injection, 2 weeks after STZ, and the effects were monitored up to 14 weeks after STZ (long-lasting effects) (a) or few hours (3 to 72 h) after the injection (short-term effects) (b). (c,d) Mice received two repeated hASC or hASC-CM administrations 2 and 6 weeks after STZ. Long-lasting effects (c) and short-term effects after the second administration (d). (e,f) Mice received a single hASC or hASC-CM injection, 6 weeks after STZ. Long-lasting effects (e) and short-term effects (f). (g) Effect of hASC-CM and CM obtained from human fibroblasts (hF-CM), administered 2 weeks after STZ. Data represent mean ± SEM of 6–8 mice per group. Two-way ANOVA followed by Bonferroni’s test was used for multiple comparisons. ***p < 0.001 vs CTR; °°°p < 0.001 vs STZ; #p < 0.05, ##p < 0.01, ###p < 0.001 vs STZ + hASC; §§§p < 0.001 vs STZ + hASC-CM; £££p < 0.001 vs. W6. (h,i) effect of hASC and hASC-CM on thermal allodynia (h) and hyperalgesia (i). Treatments were performed 2 weeks after STZ and cold allodynia and hot plate thesholds were evaluated after 3, 24, 72 hours and 1 week after treatments. Values are mean ± SEM of 6 mice per group, and were compared with Mann–Whitney U-test (cold allodynia) and two-way ANOVA followed by Bonferroni’s test for multiple comparisons (thermal hyperalgesia). *p < 0.05, ***p < 0.001 vs CTR; °p < 0.05, °°°p < 0.001 vs STZ.
