Figure 4
hASC and hASC-CM maintain a correct pro- and anti-inflammatory cytokine balance in sciatic nerves, DRG and spinal cord of STZ mice. IL-1β, IL-6, TNFα and IL-10 protein content in nervous tissues was evaluated by ELISA and reported as pg cytokine/mg total protein. (a–d) IL-1β (a) IL-6 (b), TNF-α (c) and IL-10 (d) in sciatic nerve, DRG and spinal cord of STZ mice treated 2 weeks after STZ with hASC or hASC-CM; cytokines were evaluated after 1 week from treatments. (e,f) IL-1β (e) and IL-10 (f) levels in spinal cord, measured 14 weeks after STZ in animals treated with hASC or hASC-CM either 2 weeks (W2) or 6 weeks (W6) after STZ. Data represent mean ± SEM of 6 mice per group. One-way ANOVA was used for statistical evaluation, followed by Bonferroni’s post hoc test for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001 vs CTR; °p < 0.05, °°p < 0.01, °°°p < 0.001 vs STZ; ###p < 0.001 vs STZ + hASC.
