Figure 3
Cone opsin trafficking is impaired in Tmem30a cone mutants. (A) Retina cryosections from P16 control and mutant littermates were immunostained with M opsin (green), PNA (red) and DAPI (blue). In the control retina, M opsin localized to the outer segment of cone cells. However, in mutant cone cells, M opsin accumulated in the inner segment and in cell bodies (arrows). (B) Quantification of cone cells with mislocalized M opsin. Cells were counted on 100μm ONL segment at 500 μm to the optic nerve. N = 6. ***P < 0.001. (C) Retina whole mounts from control (WT) and Tmem30a cone mutant (TmCone KO) littermate mice were immunostained with an antibody to label M opsin. Scale bar is 500 μm. In the wild-type retina, M cone cells were evenly spaced (C3). In the mutant retina, the number of M cone cells was similar. However, the cone outer segment was shorter (C4). Scale bar: 25 μm.
