Figure 5
Retinal degeneration in Tmem30a inducible knockout mutants. Paraffin sections of retinas from P25 (A,B,C. 5 days after induction), P27 (D,E,F. 7 days after induction) and P30 (G,H,I, 10 days after induction) were stained with H&E. Quantification of outer nuclear layer (ONL) nuclei revealed that the mutant ONL was reduced to approximately 50% of the control (F) at P27. At P30, the ONL was reduced to 2–3 cells per row in the mutant retina, compared with 10–11 cells per row in the control retina (I). Nuclei were counted every 200 500 μm to the optic nerve. N = 6, ***P < 0.001. Scale bar: 25μm.
