Figure 6
Analysis of TMEM30A and ATP8A2 expression in the retina of Tmem30a inducible knockout mutant mice by immunofluorescence microscopy. (A) Immunofluorescence labeling of retina cryosections from control (WT) and mutant (KO) littermates at P25 (5 days after induction) and P27 (7 days after induction) using TMEM30A antibody (red) and DAPI (blue). (B) Western blot analysis of TMEM30A in wild-type (WT) and knockout mice using TMEM30A antibody. GAPDH was used as a loading control. Quantification of TMEM30A revealed that expression of TMEM30A was decreased to 21% of that of control. Gel picture was cropped to save space. Full gel picture was listed as Figure S11. (C) Western blot analysis of ATP8A2 in wild-type (WT) and knockout mice using ATP8A2 antibody. Actin was used as a loading control. Quantification of ATP8A2 in western blotting data in (C) revealed that at P25, expression of ATP8A2 was decreased to 79% of that of control. At P27 (7 days after induction), ATP8A2 expression was further reduced to 57% of that of control. Gel picture was cropped to save space. Full gel picture was listed as Figure S12. ***P<0.001. (D) Immunofluorescence labeling of retina cryosections in control (WT) and mutant (KO) littermates at P25 (5 days after induction) and P27 (7 days after induction) using ATP8A2 antibody (green) and DAPI (blue). ATP8A2 mislocalized to the inner segment and cell bodies of retina in the mutant (KO). Scale bar: 25 μm.
