Figure 7
Increased apoptosis in Tmem30a inducible knockout mutants. (A) Immunofluorescence labeling of retina cryosections from P25 (5 days after induction) and P27 (7 days after induction) control and mutant littermates with the TUNEL labeling kit. TUNEL staining is shown in green and DAPI in blue. There were no TUNEL positive cells in the control sections. At P25, 22 TUNEL positive cells were observed in 500 μm segment in mutant mice. At P27, 82 TUNEL positive cells were observed in 500 μm segment in mutant mice. N = 6. (C,D) Immunofluorescence labeling of retina cryosections from P25 and P27 control and mutant littermates with cleaved caspase 3 (green) and DAPI (blue). Caspase 3 staining positive cells increased dramatically at both P25 and P27 in mutant mice. N = 6. P<0.001. Scale bar: 25μm.
