Table 1 Mitotic timings in mouse embryos injected with cyclin A2-YFP.

From: Delayed APC/C activation extends the first mitosis of mouse embryos

Experimental conditions

Total no.

Time between NEBD and cyclin A2 degradation (min; median (Q1; Q3))

Time between NEBD and anaphase (min; median (Q1; Q3))

1-cell

control

30

39.0 (30.0; 54.0)

111.0 (99.8; 117.0)

reversine

31

3.0 (0.0; 19.5)b

99.0 (93.0; 108.0)

wt Plk1 OE

22

15.0 (0.0; 26.3)a

108.0 (105.0; 117.0)

BI2536

38

—

—

2-cell

control

37

0.0 (0.0; 0.0)b,c,e

57.0 (51.0; 63.0)b,d,f

  1. ap < 0.05 comparing to control 1-cell embryos,
  2. bp < 0.001 comparing to control 1-cell embryos,
  3. cp < 0.05 comparing to reversine-treated 1-cell embryos,
  4. dp < 0.001 comparing to reversine-treated 1-cell embryos,
  5. ep < 0.05 comparing to 1-cell embryos over-expressing wt Plk1,
  6. fp < 0.001 comparing to 1-cell embryos over-expressing wt Plk1.
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