Table 2 Mitotic timings in mouse embryos injected with cyclin B1-Ruby.
From: Delayed APC/C activation extends the first mitosis of mouse embryos
Experimental conditions | Total no. | Time between NEBD and cyclin B1 degradation (min; median (Q1; Q3)) | Time between NEBD and anaphase (min; median (Q1; Q3)) | |
|---|---|---|---|---|
1-cell | control | 55 | 48.0 (40.5; 52.5) | 96.0 (87.0; 105.0) |
reversine | 57 | 39.0 (30.0; 45.0)b | 87.0 (81.0; 96.0) | |
wt Plk1 OE | 38 | 28.5 (24.0; 33.0)a | 78.0 (75.0; 92.3)a | |
kd Plk1 OE | 11 | 45.0 (39.0; 51.0) | 87.0 (82.5; 99.0) | |
BI2536 | 12 | 93.0 (83.3; 100.5)b | — | |
BI2536 + reversine | 15 | 90.0 (87.0; 103.5)b | — | |
cyclin A2 OE (0.07 µg/µl) | 41 | 48.0 (45.0; 51.0) | 115.5 (105.8; 128.3)a | |
cyclin A2 OE (0.2 µg/µl) | 12 | 54.0 (44.3; 60.8) | 117.0 (111.0; 135.0)e | |
cyclin A2 OE (0.4 µg/µl) | 10 | 54.0 (51.8; 67.5) | — | |
2-cell | control | 31 | 18.0 (15.0; 24.0)a,c | 76.5 (69.8; 81.0)a,d |
