Figure 3

TMSB4X has higher RNA or protein expression level in tumor samples than in normal adjacent tissues, which was demonstrated in 3 independent HNSCC cohorts. (A,B) Showed the expression changes of TMSB4X in 35 NT-paired samples of KVGH cohort by RT-PCR and real-time PCR, respectively. (C) TMSB4X (216438_s_at) expression in 23 NT-paired HNSCC cohort in microarray dataset, GSE31056. (D) IHC staining in tissue microarray from 31 NT-paired HNSCC samples of TMUH cohort, representative 3 NT-paired images. (E) TMSB4X IHC expression was scored by multiplying intensity and percentage. The P value was calculated by paired t-test.