Figure 1
Nm23-H1 and hnRNPA2/B1 overexpressed in lung cancer are recruited to the 5′-UTR of Sp1 mRNA. (A) In vitro transcribed pGEM-Sp1-5′-UTR RNA was used as a probe and was incubated with the total cell lysates of HeLa cells. The pulldown mixtures were analyzed using sliver staining and LC MS/MS. (B) Samples were harvested from cells with GFP-Nm23-H1 or myc-hnRNPA2/B1 overexpression for IP with antibodies against GFP or myc. RNA fragments were isolated from IP samples for RT-PCR. (C) Samples were collected from cells with GFP or GFP-Nm23-H1 expression for IP with anti-Nm23-H1 (a) or anti-GFP (b). IP samples were analyzed by Western blotting with antibodies against the indicated proteins. (D) The levels of Sp1, Nm23-H1and hnRNPA2/B1 in the various cancer cell lines were studied by Western blotting with antibodies against indicated proteins. (E,F) The levels of Sp1, Nm23-H1and hnRNPA2/B1 in mice with KrasG12D- and EGFRL858R-induced lung cancer were studied using IHC staining (E) and Western blotting (F). After three independent experiments, the levels were quantified and statistically analyzed with t-test, *p < 0.05; **p < 0.01; ***p < 0.005.
