Figure 5 | Scientific Reports

Figure 5

From: Nm23-H1-stabilized hnRNPA2/B1 promotes internal ribosomal entry site (IRES)-mediated translation of Sp1 in the lung cancer progression

Figure 5

The role of Nm23-H1-mediated Sp1 expression in the epithelial and mesenchymal transition of lung cancer cells. (A) Morphology of CL1-5 cells with Nm23-H1 knockdown (a) or overexpression (b) was observed with a microscope (20x), and the samples harvested from GFP-Nm23-H1-over expressed cells were analyzed by Western blotting with antibodies against the indicated proteins (c). (B,C) The distribution of F-actin in the knockdown Nm23-H1 (B) or hnRNPA2/B1 (C) in CL1-5 and H1299 cell lines was studied by immunostaining with anti-F-actin antibodies (a). The EMT-related proteins were studied by Western blotting with antibodies against the indicated proteins (b). (D) Cell morphology was observed in Nm23-H1 knockdown cells with GFP or GFP-Sp1 overexpression (a), and various EMT-related proteins were analyzed by Western blotting with antibodies against the indicated proteins (b).

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