Figure 2
From: Putrescine independent wound response phenotype is produced by ODC-like RNAi in planarians
Morphometric analysis of head and tail blastemas after ODC RNAi. (A) Schematic representation of the experimental set-up. Numbers indicate the day. Amputation was performed in the neck region. Regeneration rate was assessed by morphometric analysis cutting the animals in the neck region 7, 11 and 14 days after the first dsRNA injection and analyzing blastema size 4 days after the cut. (B) Morphometric analysis of head blastemas. Each bar is the mean ± s.d. of two independent experiments (each including 15 different specimens), normalized versus the corresponding control, to which an arbitrary value of 100% was attributed. Light blue line indicates the level of controls. Asterisks indicate cases in which a p value below 0.01 was obtained comparing areas measured in control and RNAi animals in both independent experiments. (C) Representative images of planarian fragments regenerating a new head 4 days after cut. Blastemal region is marked by a dotted yellow line. (D) Morphometric analysis of tail blastemas. Each bar is the mean ± s.d. of two independent experiments (each including 15 different specimens), normalized versus the corresponding control, to which an arbitrary value of 100% was attributed. Light blue line indicates the level of controls. Asterisks indicate cases in which a p value below 0.01 was obtained comparing areas measured in control and RNAi animals in both independent experiments. (E) Representative images of planarian fragments regenerating a new tail, 4 days after cut. Blastemal region is marked by a dotted yellow line. Scale bar corresponds to 250 μm.
