Figure 1 | Scientific Reports

Figure 1

From: Overcoming the plasticity of plant specialized metabolism for selective diterpene production in yeast

Figure 1

Diagrammatic representation of the biosynthetic pathway of labdane-type diterpenes and its reconstruction in yeast. Starting from the common precursor geranylgeranyl diphosphate (GGPP), different class II diTPSs synthesize copalyl diphosphate (CPP) or 8-hydroxy-copalyl diphosphate (8-OH-CPP). Miltiradiene synthase (MilS) can accept either CPP or 8-OH-CPP to produce miltiradiene or manoyl oxide, respectively (refs 11, 20). Subsequently, the promiscuous enzyme, CYP76AH24, oxidizes miltiradiene, abietatriene, ferruginol and manoyl oxide, to synthesize the corresponding oxygenated molecules in position C-12 or C-11 (the biosynthetic pathways are described in details in the main text). Different CYPs perform additional oxidations to produce carnosic and/or pisiferic acid (CYP76AK6), or salviol (CYP71BE52). Arrows describe the fluxes through the yeast reconstructed pathway before (blue arrows) or after engineering (red arrows) for the specific production of pisiferic acid or salviol (Figure produced using ChemDraw Professional 15.1 and Microsoft Powerpoint).

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