Table 2 Overview of carnosic acid yield improvement.
strain | protein expressed | linker | carnosic acid yield (mg/L of culture) | fold improvement (from previous step) | fold improvement (total) |
|---|---|---|---|---|---|
AM119-4 | CYP76AH24wt | 3XGS | 1.02 ± 0.08 | — | — |
AM119-4 | CYP76AH24wt | GSG | 2.18 ± 0.15 | 2.13 | 2.13 |
AM141 | CYP76AH24wt | GSG | 9.15 ± 0.50 | 4.19 | 9.33 |
AM141 | CYP76AH24wt + CPR(PTPI1) | GSG | 18.09 ± 0.82 | 1.97 | 17.73 |
