Table 3 Kinetic parameters of the engineered CYP76AH24 variants.

From: Overcoming the plasticity of plant specialized metabolism for selective diterpene production in yeast

Oxidized carbon

Substrate

Enzyme

k cat (min−1)

K M (μM)

k cat/K M (× 103 min−1 M−1)

Source

C-12

abietatriene

CYP76AH24

5.51 ± 0.71

20.62 ± 6.12

267.2

ref. 19

CYP76AH24(V296L)

6.24 ± 0.93

20.03 ± 6.97

311.5

this study

CYP76AH24(F112L)

9.69 ± 1.31

15.67 ± 5.53

618.4

this study

C-11

ferruginol

CYP76AH24

6.11 ± 0.45

45.84 ± 9.37

133.3

ref. 19

C-2α

ferruginol

CYP71BE52

0.04 ± 0.002

3.96 ± 0.72

10.1

ref. 19

C-20

ferruginol

CYP76AK6

1.43 ± 0.21

20.81 ± 7.76

68.7

ref. 19

Back to article page