Table 1 Cortical (Ct) bone parameters of the tibial midshaft, determined by in vivo microCT at days 0, 5, 10 and 15 in mice exposed to axial compression of 900 µɛ (left tibia dynamically loaded, right tibia nonloaded control) (mean ± SD); ANOVA for day 15: indicates an effect of (a) genotype, (b) age, (c) loading, (d) genotype & age, (e) genotype & loading, (f) age & loading p < 0.05.

From: Sost deficiency led to a greater cortical bone formation response to mechanical loading and altered gene expression

Outcome

LC mice

Sost KO mice

10 wk old

26 wk old

10 wk old

26 wk old

Control

Loaded

Control

Loaded

Control

Loaded

Control

Loaded

Day 0

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

Imax (mm4)

0.06 ± 0.01

0.06 ± 0.01

0.07 ± 0

0.07 ± 0.01

0.13 ± 0.02

0.14 ± 0.02

0.22 ± 0.01

0.22 ± 0.01

Imin (mm4)

0.04 ± 0.01

0.04 ± 0

0.06 ± 0

0.06 ± 0

0.11 ± 0.01

0.11 ± 0.01

0.18 ± 0.01

0.19 ± 0.01

Ct.Ar (mm²)

0.46 ± 0.02

0.45 ± 0.03

0.57 ± 0.02

0.55 ± 0.03

0.85 ± 0.05

0.85 ± 0.06

1.25 ± 0.05

1.27 ± 0.06

T.Ar (mm²)

0.95 ± 0.05

0.96 ± 0.05

1.05 ± 0.06

1.05 ± 0.04

1.41 ± 0.09

1.42 ± 0.08

1.75 ± 0.03

1.75 ± 0.04

Ct.Ar/T.Ar (mm²/mm²)

0.48 ± 0.01

0.46 ± 0.01

0.54 ± 0.02

0.52 ± 0.01

0.6 ± 0.02

0.6 ± 0.02

0.72 ± 0.03

0.72 ± 0.02

Ct.Th (µm)

158 ± 5

152 ± 6

185 ± 7

184 ± 8

254 ± 13

250 ± 15

337 ± 16

346 ± 19

Ct.vTMD (mg HA/cm3)

1263 ± 13

1239 ± 21

1316 ± 19

1320 ± 13

1268 ± 16

1263 ± 19

1309 ± 8

1326 ± 24

Day 5

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 4)

(n = 4)

Imax (mm4)

0.06 ± 0.01

0.06 ± 0.01

0.07 ± 0

0.07 ± 0.01

0.13 ± 0.01

0.14 ± 0.02

0.21 ± 0.01

0.22 ± 0.02

Imin (mm4)

0.04 ± 0.01

0.04 ± 0.01

0.06 ± 0.01

0.06 ± 0

0.11 ± 0.01

0.11 ± 0.01

0.18 ± 0.01

0.19 ± 0.02

Ct.Ar (mm²)

0.47 ± 0.04

0.46 ± 0.03

0.57 ± 0.02

0.55 ± 0.03

0.86 ± 0.06

0.86 ± 0.07

1.24 ± 0.05

1.29 ± 0.08

T.Ar (mm²)

0.98 ± 0.08

0.97 ± 0.05

1.09 ± 0.03

1.06 ± 0.04

1.39 ± 0.06

1.41 ± 0.08

1.72 ± 0.04

1.75 ± 0.08

Ct.Ar/T.Ar (mm²/mm²)

0.48 ± 0.01

0.47 ± 0.01

0.52 ± 0.01

0.52 ± 0.01

0.61 ± 0.03

0.61 ± 0.03

0.72 ± 0.03

0.74 ± 0.01

Ct.Th (µm)

160 ± 7

156 ± 5

184 ± 6

183 ± 7

259 ± 20

255 ± 20

342 ± 23

352 ± 24

Ct.vTMD (mg HA/cm3)

1264 ± 20

1234 ± 19

1314 ± 19

1316 ± 7

1270 ± 34

1261 ± 16

1313 ± 8

1363 ± 27

Day 10

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

Imax (mm4)

0.06 ± 0.01

0.06 ± 0.01

0.08 ± 0.01

0.07 ± 0.01

0.13 ± 0.02

0.15 ± 0.02

0.21 ± 0.02

0.23 ± 0.03

Imin (mm4)

0.04 ± 0.01

0.04 ± 0.01

0.06 ± 0.01

0.06 ± 0.01

0.11 ± 0.01

0.12 ± 0.01

0.18 ± 0.01

0.19 ± 0.02

Ct.Ar (mm²)

0.48 ± 0.03

0.47 ± 0.03

0.57 ± 0.03

0.55 ± 0.03

0.86 ± 0.06

0.91 ± 0.07

1.25 ± 0.06

1.29 ± 0.06

T.Ar (mm²)

0.98 ± 0.07

0.96 ± 0.05

1.09 ± 0.04

1.06 ± 0.04

1.41 ± 0.06

1.46 ± 0.06

1.72 ± 0.07

1.76 ± 0.09

Ct.Ar/T.Ar (mm²/mm²)

0.49 ± 0.01

0.49 ± 0.01

0.52 ± 0.01

0.52 ± 0.01

0.61 ± 0.02

0.62 ± 0.03

0.72 ± 0.02

0.73 ± 0.02

Ct.Th (µm)

164 ± 6

163 ± 5

183 ± 7

184 ± 5

260 ± 17

264 ± 21

340 ± 15

347 ± 18

Ct.vTMD (mg HA/cm3)

1283 ± 22

1252 ± 27

1333 ± 35

1318 ± 11

1262 ± 16

1275 ± 25

1315 ± 6

1325 ± 11

Day 15

(n = 6)

(n = 6)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 7)

Imax (mm4) a, b, c, d, e

0.06 ± 0.01

0.06 ± 0

0.07 ± 0.01

0.07 ± 0.01

0.14 ± 0.02

0.15 ± 0.02

0.22 ± 0.01

0.22 ± 0.02

Imin (mm4) a, b, c, d, e

0.04 ± 0.01

0.05 ± 0

0.06 ± 0.01

0.06 ± 0.01

0.11 ± 0.01

0.12 ± 0.01

0.18 ± 0.01

0.19 ± 0.02

Ct.Ar (mm²) a, b, c, d, e

0.49 ± 0.03

0.5 ± 0.02

0.56 ± 0.02

0.55 ± 0.03

0.87 ± 0.06*

0.94 ± 0.06

1.24 ± 0.06*

1.3 ± 0.06

T.Ar (mm²) a, b, c, e

0.97 ± 0.05

0.99 ± 0.04

1.08 ± 0.04*

1.06 ± 0.04

1.42 ± 0.07

1.46 ± 0.05

1.74 ± 0.05

1.75 ± 0.06

Ct.Ar/T.Ar (mm²/mm²) a, b, c, d, e

0.5 ± 0.01

0.5 ± 0.01

0.51 ± 0.01

0.52 ± 0.01

0.61 ± 0.02*

0.64 ± 0.03

0.71 ± 0.03*

0.74 ± 0.02

Ct.Th (µm) a, b, c, d, e

168 ± 6

170 ± 3

181 ± 7

183 ± 6

260 ± 18*

278 ± 19

328 ± 22*

357 ± 28

Ct.vTMD (mg HA/cm3) a, b, e

1279 ± 11*

1255 ± 13

1321 ± 14*

1310 ± 9

1255 ± 17*

1273 ± 26

1315 ± 10*

1330 ± 8

  1. Asterisk indicates a significant difference between loaded and control tibiae for each age and genotype (Paired t-test; p < 0.05).
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