Table 2 Endocortical (Ec) and periosteal (Ps) bone parameters of the tibial midshaft determined by dynamic histomorphometry, based on fluorochrome injections administered on days 3 and 12.

From: Sost deficiency led to a greater cortical bone formation response to mechanical loading and altered gene expression

 

LC mice

Sost KO mice

10 wk old

26 wk old

10 wk old

26 wk old

Control

Loaded

Control

Loaded

Control

Loaded

Control

Loaded

Outcome

(n = 7)

(n = 7)

(n = 7)

(n = 7)

(n = 6)

(n = 7)

(n = 7)

(n = 7)

Ec.sLS/BS (%) b, f

20.6 ± 7

11.9 ± 4.6

28.1 ± 7.4*

38.7 ± 10.1

23.6 ± 20.4

7.3 ± 7.6

27.7 ± 15.4

23.5 ± 9.3

Ec.dLS/BS (%) a, b, c, e

53.1 ± 11.3*

68.2 ± 7.7

19 ± 10

20.2 ± 10.8

66.5 ± 19.8*

84.6 ± 9.2

33.8 ± 6.3*

55.3 ± 13.5

Ec.MS/BS (%) a, b, c

63.5 ± 10.9*

74.2 ± 8

33.1 ± 9.3

39.5 ± 9.5

78.3 ± 11.4*

88.2 ± 7.7

47.6 ± 8.5*

67.1 ± 10.8

Ec.MAR (μm/day) a, b, c

1.21 ± 0.21*

1.5 ± 0.21

0.94 ± 0.22

1.11 ± 0.2

1.22 ± 0.16*

1.61 ± 0.15

1.24 ± 0.41

1.61 ± 0.38

Ec.BFR/BS (μm³/μm²/day) a, b, c, e

0.76 ± 0.12*

1.11 ± 0.19

0.32 ± 0.12

0.45 ± 0.17

0.94 ± 0.08*

1.42 ± 0.16

0.57 ± 0.13*

1.08 ± 0.29

Ps.sLS/BS (%) a, e

25.6 ± 12

28.7 ± 14

21.3 ± 4.8

24.9 ± 16.9

49.4 ± 25.6

12.4 ± 13.2

35 ± 8.2

37.1 ± 10.7

Ps.dLS/BS (%) a, b, c, e

13.3 ± 7.5

19.6 ± 13.2

1.9 ± 1.6*

4.8 ± 3

13.4 ± 7.5*

83.2 ± 19.4

16 ± 10.2*

59.1 ± 13.8

Ps.MS/BS (%) a, b, c, e

22.4 ± 13.2*

34 ± 9.9

11.9 ± 3.6

16.5 ± 8.2

33.6 ± 15.4*

89.4 ± 12.8

31.2 ± 11.5*

77.7 ± 8.7

Ps.MAR (μm/day) a, b, c, e

0.92 ± 0.33

0.89 ± 0.31

0.57 ± 0.13

0.72 ± 0.22

0.95 ± 0.19*

1.63 ± 0.1

0.63 ± 0.08*

1.09 ± 0.11

Ps.BFR/BS (μm³/μm²/day) a, b,c, e, f

0.28 ± 0.17

0.32 ± 0.16

0.07 ± 0.02

0.13 ± 0.09

0.34 ± 0.21*

1.46 ± 0.23

0.21 ± 0.08*

0.84 ± 0.09

  1. Mice were exposed to axial compression of 900µɛ (left tibia dynamically loaded, right tibia nonloaded control) (mean ± SD); ANOVA: indicates an effect of (a) genotype, (b) age, (c) loading, (d) genotype & age, (e) genotype & loading, (f) age & loading, p < 0.05. Asterisk indicates a significant difference between loaded and control tibiae for each age and genotype (Paired t-test; p < 0.05).