Table 2 Primers used for PCR analysis.

From: BHX, a novel pyrazoline derivative, inhibits breast cancer cell invasion by reversing the epithelial-mesenchymal transition and down-regulating Wnt/β-catenin signalling

Gene

Primer sequence

Forward (5′-3′)

Reverse (5′-3′)

SNAIL

TAGCGAGTGGTTCTTCTGCG

GGGCTGCTGGAAGGTAAACT

Vimentin

GAGAACTTTGCCGTTGAAGC

GCTTCCTGTAGGTGGCAATC

β-catenin

GGCAGCAACAGTCTTA

GTCTCAGGGAACATAGC

E-cadherin

TGCCCAGAAAATGAAAAAGG

GTGTATGTGGCAATGCGTTC

GAPDH

GAAGGTGAAGGTCGGAGTC

GAAGATGGTGATGGGATTTC

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