Figure 6

Combination of NCTD with CLSO arrests tumor growth in vivo. (a) Hepal-1 cells treated with the combination of NCTD and CLSO. Cell viability was assayed (n = 6 per group). (b) Microscopic view of HCC tumor tissue in mice. (c) Isolated tumor volume and Tumor weight from the Hepal-1 mouse HCC model. (d) Cleaved caspase-3 in tumor tissue lysates from vehicle and NCTD, CLSO alone or combination-treated mice were detected by immunohistochemistry analysis. ^*P < 0.05; ^**P < 0.01, vs. Vehicle, ^aP < 0.05; ^aaP < 0.01 vs. NCTD alone, ^bP < 0.05; ^bbP < 0.01 vs. CLSO alone. One-way ANOVA, post hoc comparisons, Tukey’s test. Columns, means; error bars, SDs.