Figure 1
Marked differences in parasitaemia between WT and PbNK65 hrfΔ-infected mice during blood stage development. Blood-stage parasitemia and survival (Kaplan-Meier survival plots) of C57BL/6 mice after (A) i.p. injection of 105 iRBCs or (B) i.v. injection of 103 isolated sporozoites of WT or PbNK65 hrfΔ parasites were measured at indicated time points. After infection with WT or PbNK65 hrfΔ sporozoites, livers were collected at indicated time points and RT-qPCR analysis were used to measure (C) the kinetics of parasite load using the liver stage specific LSP-2 marker expression relative to the parasite control gene HSP70 and (D) IL-6 expression using IL-6 mRNA expression relative to mouse HPRT mRNA levels. Error bars, SEM. Data are representative of two independent experiments with 5 mice per group. (*p < 0.05; Mann Whitney test).
