Figure 4
I. SOSG fluorescence imaging in cells of Arabidopsis leaves detected by confocal laser scanning microscope in WT, WT (with catechol) and lox2 mutant. The left panel represents the Nomarski interference contrast; the middle panel represents the SOSG fluorescence and the right panel represents integral distribution SOSG fluorescence intensity following 30 min of incubation in SOSG. The fluorescence signal was measured with an excitation (λex) and emission (λem) wavelengths of 488 nm and 505–525 nm, respectively. II. The intensity of the fluorescence signal in SOSG channel of confocal images (800 × 800 pixels, taken under objective magnification 40x) was exported using FV10-ASW 4.0 Viewer software (Olympus). ¼ of the image area was chosen from the cut edge of the leaves (n = 3–5 per each variant) and brightness levels, i.e. values from 0 to 4095, obtained for each of 160 000 px. Following conversion for Microsoft Excel 2010, the data were processed and presented as mean ± standard deviation, completed by maximal signal intensity value.
