Figure 3 | Scientific Reports

Figure 3

From: Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy

Figure 3

ENT2-dependent uptake is increased by insulin receptor signaling through PI3K in rat glomeruli. ENT2-mediate uptake (10 μM adenosine, 60 seconds to 22 °C) was measured in purified glomeruli from healthy male rats exposed to 5mM or 25 mM D-glucose (D-Glc) for 24 h and supplemented with insulin the last 30 minutes. Signaling through insulin receptor and intracellular pathways were characterized by using AG1024 inhibitor of the tyrosine kinase activity of the receptor and LY294002 PI3-kinase inhibitor. Graphs represent the means ± S.D. *P < 0.05 versus 5 mM D-Glc; #P < 0.05 vs 25mM D-Glc n = 9.

Back to article page