Figure 5 | Scientific Reports

Figure 5

From: Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy

Figure 5

Upregulation of ENT2 by insulin is impaired in glomeruli from diabetic rats. (A) Freshly purified glomeruli from healthy control and diabetic rats were incubated in Tyrode’s buffer for 1 min and extracellular adenosine was quantified. The graph represents means ± S.D. from individuals determinations normalized to 1 µg of total glomerular proteins. *P < 0.01 vs control; n = 5 animals in each group. (B) Particular adenosine uptake mediated by ENT1 and ENT2 (10 μM adenosine, 60 seconds to 22 °C) was measured in purified glomeruli from healthy control male rats and diabetic rats. The graphs represent the means ± S.D. *P < 0.05 versus control; #P < 0.05 versus diabetes; n = 6 animals in each group.

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