Figure 6 | Scientific Reports

Figure 6

From: Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy

Figure 6

ENT2 is downregulated by diabetes. The protein levels of ENT1 and ENT2 were evaluated in the renal glomerulus of normal and diabetic rats following 30 days of diabetes induction. Representative western blots analysis in protein extracts from purified glomeruli from rats. Induction of α-SMA was included as marker of glomerular dysfunction by diabetes and β-actin as loading control. For western blots the membrane from each gel was cut in the pieces comprising proteins in the range of 45–70 kDa and 10–45 kDa. ENT1 and ENT2 were consecutively detected in the upper piece using an stripping procedure. Similarly, α-SMA and β-actin were consecutively detected in the piece containing lower molecular weight proteins. Graphs show means ± S.D. from quantitative results from western blot analyses. Values of normal samples were normalized to 100. *P < 0.05 vs normal; n = 5 animals in each group.

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