Figure 7 | Scientific Reports

Figure 7

From: Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy

Figure 7

Protein levels of ENT2 are downregulated by inhibition of PI3K signaling pathway in podocytes. Primary cultured podocytes were exposed to insulin and LY294002 PI3-kinase inhibitor for 48 h. Representative western blot analyses of ENT1 (A) and ENT2 (B) are shown. For western blots the membrane of each gel was cut in pieces comprising proteins in the range of 45–70 kDa and 10–45 kDa. ENT1 or ENT2 were detected in the upper piece and β-actin in the respective piece containing lower molecular weight proteins. Graphs represent the means ± S.D. *P < 0.05 versus 5 mM D-Glc; #P < 0.05 vs insulin; n = 5.

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