Figure 8 | Scientific Reports

Figure 8

From: Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy

Figure 8

Increased adenosine level is correlated with early kidney alterations in diabetes. Following 1 month from diabetes induction rats were treated with PEGylated adenosine deaminase (ADA) for four weeks to decrease adenosine levels. (A) Representative images of the Periodic Acid-Schiff (PAS) staining and the immunohistochemical detection of α-SMA and nephrin in the kidney from normal rats and diabetic rats untreated or treated with ADA are shown. Bar indicates 50µm. Original magnifications 400X. (B,C) Graphs show quantitative analysis of α-SMA and nephrin staining in rat renal samples. Each animal group contained 5 rats. A number of 20 images captured from renal slides of each rat were used to quantitative analysis using the UN-SCANIT 2.0 software. *P < 0.01 vs normal; #P < 0.01 versus diabetes; n = 5. (D) The graph represents the means of urinary protein levels in rat groups at end point follow up. *P < 0.01 vs normal; #P < 0.01 versus diabetes; n = 5.

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