Figure 3

The metabolism of IMI and IMI-5-OH reported in the larvae and media of the HR_Cyp6g1 (black) and HR_Φ86FB (red) strains. Significantly less IMI and significantly more IMI-5-OH and IMI-Ole are detected in the HR_Cyp6g1 larvae and their respective media after exposure to IMI for six hrs. More IMI-Ole than IMI-5-OH was detected in these conditions (A,B). The IMI-diol metabolite (286.03360 m/z) was detected only in the HR_Cyp6g1 media. Its identification was facilitated by to the isotopic mass difference of ≈6.0201 mass units between the ¹²C₆ and the ¹³C₆- metabolites and the presence of the chlorine isotope (³⁷Cl) (C). Exposure to IMI-5-OH revealed that HR_Cyp6g1and HR_Φ86FB strains produce and excrete equal levels of IMI-Ole (D,E). Significantly higher levels of IMI-diol were detected in the HR_Cyp6g1 media (black line) compared to the control (red line) (F). Significance (indicated with asterisks) is determined comparing the levels of the metabolites produced and excreted by the HR_Cyp6g1 strain against its HR_Φ86FB control. [The data represent the mean ± SD; (n = 3); Student-t-test: ^**P ≤ 0.01, ^*P ≤ 0.05].