Figure 3 | Scientific Reports

Figure 3

From: Hypoxic Stress Decreases c-Myc Protein Stability in Cardiac Progenitor Cells Inducing Quiescence and Compromising Their Proliferative and Vasculogenic Potential

Figure 3

Hypoxic stress does not induce cardiac progenitor cell differentiation but impairs vasculogenic potential. (A) Representative western blot images showing the expression of smooth muscle markers in CPCs at 72 and 96 hours under normoxia (black bars) and hypoxia (gray bars). Graphs represent the expression of differentiation markers determined by densitometry analysis of all blots normalized to Gapdh endogenous control (n = 5). (B) Representative images of normoxia and hypoxia-treated CPCs at 6 hours (top panels, 4X magnification) and 24 hours (lower panel, 10X magnification) after culturing on Matrigel (n = 5). Cells in 24 hour-time point images were stained with calcein-AM to indicate that they were alive by the end of the study.

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