Figure 8 | Scientific Reports

Figure 8

From: Hypoxic Stress Decreases c-Myc Protein Stability in Cardiac Progenitor Cells Inducing Quiescence and Compromising Their Proliferative and Vasculogenic Potential

Figure 8

Expression of GSK-3β under hypoxia and effect on c-Myc expression. (A) Representative western blot image showing increased expression of inhibited GSK-3β (Ser9) under hypoxia (gray bars) relative to normoxia (black bars), after short stimulation with culture media. Graph represents Phospho/Total GSK-3β expression determined by densitometry analysis of all blots (n = 4, *p < 0.05, **p < 0.007). (B) Effect of GSK-3β inhibition by TCS2002 on CPCs proliferation under normoxia and hypoxia (n = 4, *p < 0.05, **p < 0.001). (C) Effect of GSK-3β inhibition by TCS2002 (gray bars) on c-Myc expression in CPCs cultured under normoxia and hypoxia relative to vehicle control (black bars). Inhibitor was tested at 50 and 100 nM. Graph represents c-Myc expression determined by densitometry analysis of all blots normalized to Actin endogenous control (n = 4, *p < 0.05).

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