Figure 7

Different formulations of liposomes have different levels of adhesion and extravasation after 48 h of co-culture of HBTAEC and MDA-MB-231 tumor cells. Functionalized liposomes with antibodies to E-selectin (a-E-selectin) have significantly higher adhesion in the vascular compartment and extravasation into the tumor compartment (Figure S3). Dual targeting to both E-selectin and ICAM-1further increased binding when compared to E-selectin single targeting (positive control, Figure S3). Extravasation of E-selectin targeted liposomes was significantly increased when compared to non-targeted liposomes, but no significant difference in extravasation was observed between single and dual targeted liposomes. Data are presented as mean ± SEM (n = 3). *Significant difference by ANOVA.